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Bovine IgM ELISA Kit Cat#8010 | |
Kit Contents
| Anti-Bovine IgG coated strip plate (8 wells x 12 strips) 80112. Bovine IgG Std. A (0 ng/ml), 0.25 ml 80123. Bovine IgG Std. B (25 ng/ml), 0.25 ml 80134. Bovine IgG Std. C (50 ng/ml), 0. 25 ml 80145. Bovine IgG Std. D (100 ng/ml), 0. 25 ml 80156. Bovine IgG Std. E (250 ng/ml), 0. 25 ml 80167. Bovine IgG Std. F (500 ng/ml), 0. 25 ml 80178. Bovine IgG Std. G (1000 ng/ml), 0. 25 ml 80189. Sample Diluent (20x), 10ml SD-20B10. Wash Buffer (100X), 10 ml WB-10011. Anti-Bovine IgG-HRP Conjugate (100x), 0.12 ml 801912. TMB Substrate, 12 ml 8009113. Stop solution, 12 ml 8010114. Instruction Manual M-8010 |
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates | |
Samples | Serum. Plasma, Culture medium and other fluids may be adapted |
Crossreactivity | Bovine IgG ELISA kit cross reactivity was tested with the following animal sera (IgG) at dilutions of 1:10k: Significant crossreactivity was observed with Rabbit (2.2%) serum IgG. Human, Rat, Mouse, Goat, Sheep, PIG, FBS, Guinea Pig, Hamster, Monkey, and Chicken serum IgG had less than 1% crossreactivity |
Reactivity | Cow/Bovine |