Probetex，Inc公司成立于1997年，是一家為生物科研研究提供服務(wù)的CRO 公司。公司專注于6個研究領(lǐng)域：1）成人和胚胎腎細胞系的細胞庫2）生產(chǎn)和銷售綿羊腎毒性抗體以誘導抗腎小球基底膜（抗GBM）腎小球腎炎，膜性（抗Fx1A）腎病和系膜增生性（抗Thy1）腎小球腎炎。3）腎病模型研究的組織產(chǎn)品4）組織病理學服務(wù)，提供常規(guī)組織學，免疫組織化學，電子顯微鏡和圖像分析; 5）提供以上腎病以及20多種急性、慢性實驗性腎病、心腦血管和肺病的其他模型的構(gòu)建服務(wù)。6）其他研究領(lǐng)域，比如：再生醫(yī)學、血管炎新模型以及其他罕見疾病模型的構(gòu)建和評價服務(wù)。

特色產(chǎn)品：

綿羊抗大鼠腎小球基底膜血清（PTX-001AGBM）

Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum

--抗GBM型腎炎模型誘導

應(yīng)用說明：(實驗前請仔細閱讀實驗流程)

每瓶血清含有足夠的抗體用于誘導至少25只大鼠*（175-200 g體重）被動抗GBM腎炎模型。

免疫抗GMB血清：麻醉大鼠，尾靜脈注射抗GMB血清超過15秒，疾病的產(chǎn)生依賴于免疫劑量，依照免疫劑量*打入體內(nèi)很關(guān)鍵（推薦用量：0.4-0.5ml/100g體重）。由于不同批次抗血清差異，大鼠來源，以及實驗要求的不同，我們建議進行劑量-反應(yīng)研究，具體的免疫劑量和疾病的嚴重程度，需要根據(jù)每個不同批次血清和產(chǎn)生疾病的嚴重程度，選擇合適的免疫劑量。

腎病研究：

1）異源性疾病：免疫抗GBM (anti GBM)3-5天后，免疫熒光檢測顯示綿羊IgG在腎小球基底膜內(nèi)呈線狀沉積。大鼠c3也呈線性分布，24小時后出現(xiàn)蛋白尿。

2）自身疾病：免疫抗GBM抗體8-10天后，檢測大鼠蛋白尿增加，同時大鼠腎小球IgG(自身抗體) 呈線狀沉積，自體疾病變得明顯。（自體）免疫組化呈線性模式的IgG（圖1）。到3周時，蛋白尿有望達到200毫克/24小時，腎小球病變（圖2）明顯，隨著時間的推移，嚴重程度增加，導致腎小球硬化。

圖1.免疫熒光檢測顯示免疫抗GBM3周后腎小球毛細血管壁大鼠IgG免疫沉積的。

圖2.免疫組化染色顯示免疫抗GBM 3周后新月體形成、系膜細胞增生、毛細血管變形。

相關(guān)產(chǎn)品：

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